Bridges to the Baccalaureate Program Directed Research 2012

NIH City College of San Francisco/Skyline College/Cañada College/San Francisco State University

Bridges to Baccalaureate Program
Directed Research Program
Summer 2012
Instructors: Chantilly Apollon, CCSF, & Sally Pasion, SF State

Click here to go to Part I (Weeks 1 - 4): Directed Research Program - Chantilly Apollon

Click here to go to Part II (Weeks 5 - 8): Directed Research Program - Sally Pasion

Monday, June 4, 2012: Orientation Day

9:00 am - 10:30 am	Introductions & Interactive Group Discussions
10:30 am - 11:15 am	Discussions on Expectations
11:15 am - 12 noon	Keeping a Lab Notebook
12 noon - 1:00 pm	lunch break
1:00 pm -2:00 pm	Searching for research articles PubMed http://www.ncbi.nlm.nih.gov/pubmed/
	Task #1 web site: http://www.nlm.nih.gov/bsd/disted/pubmed.html
	Cell and Molecular and Microbiology Online journals available by SFSU subscription
2:00 pm -2:45 pm	Discussion on Guiding Principles
2:45 pm -4:00 pm	Lab Safety
	Task #4 web site 1: http://ehs.okstate.edu/modules/index.htm?option=com_extcalendar&Itemid=59&extmode=view&extid=287
	Task #4 web site 2: http://www.youtube.com/watch?v=V8eiJ9AeCt8&feature=related
	Task #5: Scavenger Hunt

Additional Lab Safety web sites of interest:

Lab Safety Song video: http://www.youtube.com/watch?feature=player_embedded&v=WZ-1lfammjk#!

MSDS index: http://hazard.com/msds/

Guide to Gloves: http://www.hazmat.msu.edu/glove_guide/

Part II: JULY 2 - JULY 30, 2012 (note that this section is being updated)
Instructor: Sally Pasion, Ph.D. Teaching Assistant: Gary Guerrero
NOTE: The agendas below are tentative and subject to change.
Computers must be returned to stockroom by 4:00pm
Week 5: Jul 2 - 6, 2012
Week 6: Jul 9 - 13, 2012
Week 7: Jul 16 - 20, 2012
Week 8: Jul 23 - 30, 2012

click on this link to view course materials for Part I: Weeks 1 - 4 (Jun 4-Jun 29, 2012)

Week 5: Jul 2 - 6, 2012
Wednesday 7/04 is Independence Day- SFSU Campus Closure
Mon 7/02 Tue 7/03 Thu 7/05 Fri 7/06

For more information on fission yeast: Pombe.net

For more information on a career in biology: Women in Biology Internet Launch page

Monday, 7/02/12
9:00 – 9:30	Introductions, again
9:30 – 10:30	Fission yeast morphology Fission yeast cell division movie S. pombe cell division movie S. japonicus cell division movie
10:30 – 12:00	Exercise 1: Fission Yeast microscope observation
12:00 – 1:00	lunch break
1:00 – 2:00	Exercise 1: Fission Yeast microscope observation & Discussion
2:00 – 3:30	Introduction to bacteria and plasmids Streaking out bacteria on agar plate Formation of bacterial colony on agar plate
3:30 – 3:45	Exercise 2-1: Plasmid DNA inoculation
3:45 – 4:00	Lab clean-up

Tuesday, 7/03/12
9:00 – 10:00	Introduction to fission yeast/media
10:00 – 12:00	Exercise 3-1: Genotype & phenotype
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 3:45	Exercise 2-1: Plasmid DNA isolation (restreak transformants)
3:45 – 4:00	Lab clean-up

Thursday, 7/05/12
9:00 – 10:00	Discussion
10:00 – 12:00	Exercise 5-1: Media preparation
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 2:00	Exercise 5-1: Media preparation
1:00 – 3:45	Exercise 2-2: Plasmid DNA isolation
3:45 – 4:00	Lab clean-up

Friday, 7/06/12
9:00 – 10:00	UV light and DNA damage Repair of UV damage animation
10:00 – 12:00	Exercise 6-1: Yeast UV sensitivity
12:00 - 1:00	lunch break
1:00 – 3:45	Exercise 6-1: Yeast UV sensitivity
1:00 – 3:45	Discussion on scoring yeast genotype Exercise 3-2: Genotype & Phenotype
3:45 - 4:00	Lab clean-up

Week 6: Jul 9 - 13, 2012
Mon 7/09 Tue 7/10 Wed 7/11 Thu 7/12 Fri 7/13

Monday, 7/09/12
9:00 – 9:30	Meiosis
9:30 – 10:30	Exercise 7-1: Cross two yeast mutants
10:30 – 12:00	Exercise 6-2: Yeast UV sensitivity
12:00 – 1:00	lunch break
1:00 – 2:30	Exercise 6-2: Yeast UV sensitivity
2:30 – 3:45	Exercise 3-2: Genotype & phenotype
3:45 – 4:00	Lab clean-up

Tuesday, 7/10/12
9:00 – 11:00	Restriction Enzyme Digestion Exercise 4-1: Restriction Enzyme Digestion
11:00 – 12:00	Transformation
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 3:45	Exercise 10-1: Yeast Transformation
3:45 – 4:00	Lab clean-up

Wednesday, 7/11/12
9:00 – 10:30	Exercise 4-2: Restriction Enzyme Digestion
10:30 – 12:00	Exercise 3-3: Genotype & Phenotype
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 3:45	Exercise 4-2: Restriction Enzyme Digestion
1:00 – 3:45	Exercise 8-1: Independent UV Experiment
3:45 – 4:00	Lab clean-up

Thursday, 7/12/12
9:00 – 11:00	Exercise 3-4: Genotype & phenotype Exercise 3-4 (Word doc) S. pombe GeneDB (useful pombe database) PubMed Online Mendelian Inheritance in Man (OMIM) Sally's web page on SFSU Online Journals SFSU Library - Search for Electronic Journals
11:00 – 12:00	Exercise 8-2: Independent UV Experiment
12:00 – 1:00	lunch break
1:00 – 3:45	Exercise 8-2: Independent UV Experiment Exercise 6-1 (Word doc) Exercise 8-3_Prep (Word doc)
1:00 – 3:45
3:00 - 3:45	Exercise 7-2: Cross two yeast mutants
3:45 – 4:00	Lab clean-up

Friday, 7/13/12
9:00 - 12:00	Exercise 8-3: Independent UV Experiment
12:00 - 1:00	lunch break
1:00 - 2:00	Exercise 8-3: Independent UV Experiment
2:00 - 3:45	Exercise 7-3: Cross two yeast mutants
3:45 - 4:00	Lab clean-up

Week 7: Jul 16 - 20, 2012

Mon 7/16 Tue 7/17 Wed 7/18 Thu 7/19 Fri 7/20

Monday, 7/16/12
9:00 – 10:00	Exercise 5-2: Media preparation
10:00 – 12:00	Exercise 8-4: Independent UV Experiment
12:00 – 1:00	lunch break
1:00 – 2:00	Exercise 5-2: Media preparation
1:00 – 2:00	Exercise 8-4: Independent UV Experiment
2:00 – 3:00	Exercise 9-2: Yeast Transformation
3:00 – 3:45	Exercise 7-3: Cross two yeast strains
3:45 – 4:00	Lab clean-up

Tuesday, 7/17/12
9:00 – 12:00	Exercise 3-4: What is your gene?
9:30 – 12:00	Exercise 4-2: Restriction Enzyme Digestion Gel A1 (jpg) Gel A1 inverted (jpg) Gel A2 (jpg) Gel A2 inverted (jpg) Gel B (jpg) Gel B inverted (jpg) Gel C (jpg) Gel C inverted (jpg)
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 3:45	Exercise 3 - Report to class
1:00 – 3:45	Exercise 4 - Report to class
3:45 – 4:00	Lab clean-up

Wednesday, 7/18/12
9:00 – 10:30	Central Dogma Copying the Code and Reading the Code (Cold Spring Harbor Lab)
10:30 – 12:00	Exercise 4 - Report to class Exercise 4-2 REPORT (ppt)
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 3:45	TBA
3:45 – 4:00	Lab clean-up

Thursday, 7/19/12
9:00 – 10:00	TBA
10:00 – 12:00	Exercise 8-5: Independent UV Experiment
12:00 – 1:00	lunch break
12:00 – 3:45	Exercise 8-5: Independent UV Experiment
12:00 – 3:45	TBA
3:45 – 4:00	Lab clean-up

Friday, 7/20/12
9:00 - 12:00	Exercise 8-6: Independent UV Experiment
12:00 - 1:00	lunch break
1:00 - 3:00	Exercise 8-6: Independent UV Experiment
2:00 - 3:45	Review of In the Family documentary Update on the filmmaker
2:00 - 3:45	Exercise 10-1: Mating type check
2:00 - 3:45	Exercise 9-2: Yeast transformation
3:45 - 4:00	Lab clean-up

Week 8: Jul 23 - 30, 2012

Mon 7/23 Tue 7/24 Wed 7/25 Thu 7/26 Fri 7/27 Mon 7/30

Monday, 7/23/12
9:00 – 12:00	Exercise 8-7: Independent UV Experiment
9:00 – 12:00	Poster Prep
12:00 – 1:00	lunch break
1:00 – 3:45	Exercise 7-5: Cross two yeast mutants
1:00 – 3:45	Exercise 8-7: Independent UV Experiment
1:00 – 3:45	Poster Prep
3:45 – 4:00	Lab clean-up

Tuesday, 7/24/12
9:00 – 12:00	Poster Prep Poster web sites: Creating Effective Poster Presentations Designing Conference Posters Tutorial on Designing Effective Posters
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 3:45	Poster Prep
3:00 – 3:45	Exercise 10-2: Mating type check
3:45 – 4:00	Lab clean-up

Wednesday, 7/25/12
9:00 – 12:00	Exercise 11-1: Yeast Growth Curve
9:00 – 12:00	Poster Prep
12:00 – 1:00	lunch break (SGP has Office Hours)
1:00 – 3:45	Exercise 11-1: Yeast Growth Curve
1:00 – 3:45	Poster Prep
3:45 – 4:00	Lab clean-up

Thursday, 7/26/12
9:00 – 12:00	Poster Prep
12:00 – 1:00	lunch break
1:00 – 3:45	Poster Prep
3:45 – 4:00	Lab clean-up

Friday, 7/27/12
9:00 – 12:00	Poster Prep
12:00 – 1:00	lunch break
1:00 – 3:00	Practice Poster Session
3:00 – 4:00	Lab clean-up

Monday, 7/30/12
9:00 – 12:00
9:00 – 12:00	Poster Prep
11:30 – 12:00	Survey
12:00 – 1:00	lunch break
1:00 – 3:45	Poster Prep
2:30 – 3:45	Exercise 7-5: Cross two yeast mutants
2:30 – 3:45	Exercise 9-3: Yeast transformation
2:30 – 3:45	Exercise 11-1: Yeast Growth Curve
3:45 – 4:00	Lab clean-up